| ORIGINAL RESEARCH |
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| Year : 2020 | Volume
: 9
| Issue : 4 | Page : 151-156 |
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Changing serotypes of rotavirus in Western India and clinical severity of diarrhoea
Kshitija Rane1, Pankhuri Kothari2, Mansi Thakur1, Dattatraya Joshi1, Nitin Kadam2, Nimain Mohanty2
1 Department of Medical Genetics & Biotechnology, MGMIHS, Kamothe, Navi Mumbai, Maharashtra, India
2 Department of Pediatrics, MGM Medical College and Hospital, Navi Mumbai, Maharashtra, India
Correspondence Address:
Nimain Mohanty
Department of Pediatrics, MGM Medical College, Navi Mumbai, Maharashtra
India
 Source of Support: None, Conflict of Interest: None
DOI: 10.4103/jpai.jpai_6_21
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Introduction: Rotavirus and Norovirus diarrhea pose highest threat to neonates and toddlers all over the world. Diarrhoea happens to be the second most common cause of under-5 mortality in India after Pneumonia. India has the highest incidence of rotavirus diarrhoea, contributing to about 1,50,000 inder-5 deaths annually. Virus serotypes do undergo constant changes, varying in different regions, thought of responding to vaccines differently. Objecive: A prospective observational study was undertaken in Kamothe, Navimumbai (India); enlisting 207 cases of acute watery diarrhoea among under-5 children to study incidence of Rotavirus and Norovirus as causal agents and their serotypes. Efforts were made to understand if virus positivity and their types were related to clinical severity of diarrhoea. Method: Stool samples were collected and tested for rotavirus and norovirus from cases of acte diarrhoea on clinical presentation and after excluding cases of dysentery on clinical presentation. 10.62% cases were found positive for rotavirus RNA by ELISA and 4.83% by Electropherotyping. Cases of acute watery diarrhoea having rotavirus positive and negative stool showed remarkable difference with diarrhoea severity assessed by Vesikary scores. Severe dehydration, stool frequency, vomiting and weight loss were associated with rotavirus infection in younger age group. Long electropherotypes were more prevalent (86.36%) and were associated with higher clinical severity of diarrhoea. P[8] was predominant (77.27%) followed by P[4] (13.63%) genotypes. Neither P[6] nor P[11] was detected. Those found positive for rotavirus in their stool samples, were negative in their serum by eletropherotyping. Only 0.48% of diarrhoea stool samples were found positive for Norovirus by ELISA. Conclusion: Rotavirus diarrhoes shown a decreasing trend in Navimumbai area as compared to previous years. Incidence of Norovirus infection was found very low. Rotavirus positive cases, particularly of long arm electropherotypes, showed clinical severity of diarrhoea as compared to short-arm types and in those found negative for rotavirus or Norovirus.
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